Simple method for DNA extraction from pancreatic juice for PCR amplification assays

Summary Conclusion: Preparation of DNA from pancreatic juice for subsequent polymerase chain reaction (PCR) is difficult, but manageable. The protocol presented offers a simple and fast solution. This method might be applicable to other complicated samples, such as saliva, wound secretions, or stool washings. Background: Of all the biological samples used for PCR amplification, pancreatic juice is the most problematic because of the presence of potential inhibitory substances and the amount of nucleases. This demands a DNA preparation procedure that is suitable for routine diagnostic PCR, and is therefore efficient and safe. This is particularly true for pancreatic juice obtained during routine endoscopy. Methods: We describe here a simple method utilizing modified phenol/chloroform extraction and precipitation directly from native pancreatic juice suitable for diagnostic PCR applications, such as oncogenes. Results: DNA could be prepared in quantitative amounts from routine endoscopic specimens. DNA could also be prepared from samples kept several days at room temperature.     

The role of fruit juice in the diet: an overview

Over recent years, consumers have shown an increasing interest in health and wellbeing, with many wanting food and drink products that are healthy and nutritious, yet fit in with busy lifestyles. Fruit juice is one beverage that has seen a dramatic increase in consumer purchasing since the 1970s, and is now consumed by nearly half the UK population. While the beneficial effects of consuming diets rich in fruit and vegetables are well documented, the specific health effects of consuming fruit juice are less so. Further, the role of fruit juice in conditions such as obesity has also recently come under scrutiny, due to questions over how liquid calories affect subsequent energy intake. Limitations in the literature in this area include a lack of studies looking at different types of fruit juices and their effects on health, as well as studies that differentiate fruit juice from fruit and vegetable intake. Consuming a diet rich in fruit and vegetables (to which fruit juice can count once towards the 5 A DAY target) can help to reduce an individual's risk of suffering from a variety of chronic diseases, and should therefore be encouraged.     

刺梨汁对骨髓抑制小鼠造血功能的调控作用

目的 探讨刺梨汁对环磷酰胺合并⁶⁰Co致骨髓抑制小鼠造血功能的影响,研究其促进血细胞上调的作用机制。方法 双抗体夹心法检测外周血象、骨髓基质中红细胞生成素（EPO）、血小板生成素（TPO）、粒细胞生长因子（GCSF）的量。结果 刺梨汁能明显改善骨髓抑制小鼠外周血象;能有效平衡微环境中TPO的量;改善EPO的表达;对骨髓抑制小鼠骨髓细胞上清中GCSF有明显的正调控作用。结论 刺梨汁能提高骨髓抑制小鼠外周血血细胞和骨髓有核细胞计数;可促进骨髓抑制小鼠骨髓细胞从G₀/G₁期进入增殖周期;并能有效调节骨髓微环境中TPO、EPO、GCSF的表达,从而促进骨髓抑制小鼠的造血功能,可作为一种治疗贫血的辅助保健药物。     

奥美拉唑与脑卒中相关性肺炎的关系研究

目的探讨无法进食经鼻饲的急性脑卒中患者给予奥美拉唑后胃液pH值、胃腔细菌定植情况以及对应激性消化道溃疡、卒中相关性肺炎（SAP）的影响。方法90例患者依据奥美拉唑应用时间随机分为三组,每8小时抽取胃液,测pH值及潜血试验。留取胃液、深部痰行细菌培养。结果三组胃液pH值、各组SAP发生率差异有统计学意义：三组应激性溃疡的发生率差异无统计学意义。90例患者中31例发生胃腔细菌定植,三组差异有统计学意义。胃腔细菌定植患者pH值与无细菌定植患者胃液pH值差异有统计学意义;发生胃腔细菌定植细菌主要为革兰阴性杆菌;17例SAP患者痰细菌培养主要为革兰阴性杆菌。17例SAP患者pH值与非SAP患者胃液pH值差异有统计学意义;急性脑卒中鼻饲患者,应用奥美拉唑时间延长,胃液pH增加,胃腔细菌定植增多,SAP发生率增加,应激性溃疡发生率下降。结论对于急性脑卒中患者应选择应用奥美拉唑时间,需监测胃液pH值,使胃液pH值保持适当范围。     

石榴多酚对心肌缺血/再灌注损伤大鼠心功能的保护作用

目的观察石榴多酚对心肌缺血/再灌注损伤(I/R)大鼠的心功能的影响,并初步探讨其可能机制。方法采用阻断大鼠左冠状动脉前降支45 min,再灌注180 min心肌缺血/再灌注损伤模型。50只雄性SD大鼠随机分为伪结扎组(Sham)、缺血/再灌注组(I/R)和石榴多酚(150、300、600 mg/kg)组。连续监测心功能变化,实验结束后检测血浆乳酸脱氢酶(LDH)、磷酸肌酸激酶(CK)、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性,TTC染色法检测心肌梗死面积。结果与伪结扎组相比,缺血/再灌后各组大鼠的心功能指标中,左室收缩压(LVSP)、左室内压最大上升/下降速率(±dp/dtmax)明显降低(P<0.01),左室最大舒张压(LVEDP)明显升高(P<0.01);与缺血再灌模型组相比,石榴多酚(150、300、600 mg/kg)组缺血再灌大鼠的心脏收缩和舒张功能(LVSP、LVEDP及±dp/dtmax)显著改善(P<0.05);LDH、CK和MDA含量降低(P<0.01),SOD活性增加(P<0.01),心肌梗死程度减轻(P<0.01)。结论石榴多酚对缺血/再灌注损伤大鼠的心功能有显著的保护作用,其机制可能与石榴多酚提高氧自由基清除能力、降低脂质过氧化损伤,减轻心肌梗死程度有关。     

内镜在慢性萎缩性胃炎诊断中的应用价值与幽门螺杆菌相关性研究

目的探讨内镜胃液pH值的检测在慢性萎缩性胃炎诊断中的应用价值及慢性萎缩性胃炎与幽门螺杆菌相关性。方法选取2008年1~12月于我院进行治疗的46例慢性萎缩性胃炎患者为研究对象,将其设为观察组（A组）,同时选取同期的46例慢性浅表性胃炎患者为对照组一组（B组）,46例健康人为对照二组（C组）,分别采用内镜对三组胃液pH值进行研究统计,并将慢性萎缩性胃炎患者的严重程度与幽门螺杆菌的阳性率进行统计比较。结果经研究发现,A组胃液pH值明显高于B组及C组,P〈0.05,B组与C组pH值比较,差异无统计学意义（P〉0.05）。且重度者幽门螺杆菌阳性率明显高于中度及轻度者,差异有统计学意义（P〈0.05）。结论内镜胃液pH值的检测在慢性萎缩性胃炎诊断中有较重要的诊断价值,且随着慢性萎缩性胃炎的加重,幽门螺杆菌的阳性率也呈上升趋势。     

药用沙棘果汁中1个新的黄酮苷类化合物

目的 对药用沙棘Hippophae rhamnoides果汁的化学成分进行研究.方法 选用大孔树脂、硅胶柱色谱、Sephadex LH-20、ODS、HPLC等色谱分离技术及HR-ESI-MS、1H-NMR、13C-NMR、HSQC、HMBC、NOESY等波谱分析技术进行分离、纯化与鉴定.结果 从药用沙棘果汁经D-10...     

Malignant potential and specific characteristics of pure main duct type intraductal papillary mucinous neoplasm

BackgroundAs the malignant potential of main duct (MD-) type intraductal papillary mucinous neoplasm (IPMN) has been discussed together with Mixed-type in most previous studies, the malignant potential of pure MD-type IPMN remains unclear. This study evaluated the specific characteristics and predictors of high-grade dysplasia (HGD) and invasive intraductal papillary mucinous carcinoma (IPMC) for pure MD-type IPMN.MethodsFrom 1,100 patients with IPMN, this study includes 387 patients that underwent surgery. We evaluated the specific characteristics of pure MD-type IPMN by comparing clinicopathological factors between MD-type (n = 79) and branch duct (BD-) type (n = 146) or Mixed-type IPMN (n = 162), and predictors of HGD/invasive IPMC in pure MD-type IPMN.ResultsThe rate of HGD/invasive IPMC was significantly higher in MD-type than in BD-type (70.9 vs. 48.6%, P = 0.001), although there was no difference between MD-type and Mixed-type IPMNs (P = 0.343). Recurrence-free survival (RFS) and disease-specific survival (DSS) of patients with MD-type were better than those of patients with Mixed-type (P = 0.008 and P = 0.009, respectively). There were no significant differences in RFS, overall survival, and DSS between patients with MD-type and patients with BD-type IPMNs. Multivariate analysis showed two independent predictors of HGD/invasive IPMC in MD-type IPMN; mural nodule height ≥5 mm (P = 0.025, odds ratio [OR]; 16.949) and carcinoembryonic antigen (CEA) level in the pancreatic juice obtained by preoperative endoscopic retrograde pancreatography ≥50 ng/ml (P = 0.039, OR; 9.091).ConclusionsMeasurement of mural nodule height and CEA in the pancreatic juice might be useful in determining surgical indication for pure MD-type IPMN, although further studies for confirmation are essential.     

星点设计-响应面法优化超声波提取石榴皮多糖的工艺及抗氧化活性研究

目的通过星点设计-响应面法优化石榴皮多糖的提取工艺及抗氧化活性。方法以石榴皮多糖的含量为指标,采用超声波辅助技术提取石榴皮多糖,用星点设计-响应面法研究了液料比、提取时间、提取温度和超声功率对提取石榴皮多糖的影响;观察了对二苯基苦基苯肼自由基(·DPPH)、羟基自由基(·OH)、过氧化氢(H2O2)的清除作用。结果超声波提取石榴皮多糖的最佳工艺为:液料比29.98(mL∶g),提取时间43.72 min,提取温度62.79℃,超声功率454.30 W,以此工艺条件得多糖提取率为7.03%;石榴皮多糖对·DPPH、·OH、H2O2有明显的清除作用。结论星点设计-响应面法用于优化提取工艺具有简便合理、稳定的特点,在优化石榴皮多糖提取条件中应用效果良好;石榴皮多糖有明显的抗氧化作用。